Team: UCAS-China

DeCaffi

Jun 14th, 2021

来自中国科学院大学的陈冠羽、刘昱辰、董可昕、黄枫凌出席了第八届中国地区iGEMer交流会（2021年8月27至29日，中国上海）。 组委会提前收到了来沪人员的意外险保单。

Team Leader

• 陈冠羽
• 刘启涵*

Contact

• C867469688 AT hotmail.com

A Food & Nutrition Project

Our project aims to remove caffeine from drinks by synthetic biology methods. To achieve this goal, we will convert caffeine into healthful theacrine through a two-step enzymatic reaction in a cell-free system. This process requires two enzymes: Cdh and CkTcS, which will be produced by our engineered bacteria modified by synthetic biology methods These two enzymes can utilize CoQ(0) and SAM to turn caffeine into theacrine. For this aim, we will also use engineered bacteria for fermentatve production of SAM. In addition, we I use the PUTR-CRP global transcrptional regulatory system to enhance the expression of target genes. All our genetically modified engineered bacteria will be strictly confined to the laboratory culture, and we will also transfer the cryo-induced suicide module relB-relE+Ptrc into them to eliminate the risk of engineered bacteria’s leaking. Finally, all substances needed in the decaffeine process will be purified, sterilized and then added to the cartridges. Through this. we can ensure that our cartridges can be used to filter drinks without causing harm to humans and the environment.

https://2021.igem.org/Team:UCAS-China
https://igem.org/Team.cgi?id=3817
http://parts.igem.org/wiki/index.php?title=Part:BBa_K3817000
UCAS-China Project Promotion video at igem.org

Team of UCAS-China present between 2021/8/28 13:00-14:30 using VooV 404 469 7665^28#3.